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goat polyclonal igg prox1  (R&D Systems)


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    Structured Review

    R&D Systems goat polyclonal igg prox1
    Goat Polyclonal Igg Prox1, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 318 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/goat+polyclonal+anti+prox1/Human+Prox1+Antibody/pmc12893340-88-43-47
    Average 95 stars, based on 318 article reviews
    goat polyclonal igg prox1 - by Bioz Stars, 2026-09
    95/100 stars

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    Immunofluorescence:

    Article Title: Lymphatics and fibroblasts support intestinal stem cells in homeostasis and injury.
    Article Snippet: All antibody dilutions were performed in Signalstain Antibody Diluent (CST, 8112L). .. The following primary antibodies were used for immunofluorescence: goat polyclonal anti-GFP (1:500, abcam, ab6673), rat monoclonal anti-LYVE1 (1:1000, Thermo Fisher Scientific, 14-0443-82), rabbit polyclonal anti-LYVE1 (1:1000, Sigma Aldrich, HPA042953), mouse monoclonal anti-Desmin (1:200, abcam, ab6322), mouse monoclonal anti-ADAMDEC1 (1:100, Thermo Fisher Scientific, 6C4), mouse monoclonal anti-EpCAM (1:4,000, Biolegend, 324201), goat polyclonal anti-Prox1 (1:400, R&D, AF2727) and rabbit polyclonal anti-RFP (1:400, Rockland 600-401-379). .. Alexa Fluor secondary antibodies, anti-goat 488, anti-mouse 488, anti-rabbit 568, anti-mouse 647, and anti-rat 647 (1:500, Thermo Fisher Scientific), were used for visualization.

    Immunocytochemistry:

    Article Title: Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells.
    Article Snippet: .. Immunocytochemistry was performed using a standard protocol and the following primary antibodies: mouse monoclonal antiNESTIN (Rat-401, 1:100; Developmental Studies Hybridoma Bank, IA, United States), rabbit polyclonal anti-SOX2 (ab15830, 1:150; Abcam, United Kingdom), rabbit polyclonal anti-brain lipid binding protein (BLBP; AB9558, 1:500; Chemicon, Hampshire, United Kingdom), mouse monoclonal antiVIMENTIN (V6630, 1:40; Sigma-Aldrich), rabbit polyclonal anti-NG2 (AB5320, 1:150; Millipore, United Kingdom), rabbit polyclonal anti-OLIG2 (AB9610, 1:200; Millipore, MA, United States), mouse monoclonal anti-OLIG2 (SAB1404798, 1:50; Sigma-Aldrich), goat polyclonal anti-PROX1 (AF2727, 1:15; R&D Systems), mouse monoclonal anti-CNPase (C5922, 1:500; Sigma-Aldrich), mouse monoclonal anti-adenomatous polyposis coli (CC1 antibody, OP80, 1:1,000; Calbiochem, La Jolla, CA, United States), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; MAB360, 1:800; Chemicon, CA, United States), mouse monoclonal anti-βIII tubulin (Tuj1, 1:100; BabCO, CA, United States), rat monoclonal anti-myelin binding protein (MBP; MAB386, 1:200, Chemicon), and chicken polyclonal anti-neurofilament heavy polypeptide (NFH; ab72996, 1:25,000; Abcam). .. Secondary antibodies raised against the appropriate host species and conjugated with Alexa Fluor 488-, Alexa Fluor 594-, and Alexa Fluor 680 dyes were used to detect primary antibodies (1:500; all from ThermoFisher scientific).

    Article Title: Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells
    Article Snippet: .. Immunocytochemistry was performed using a standard protocol and the following primary antibodies: mouse monoclonal anti-NESTIN (Rat-401, 1:100; Developmental Studies Hybridoma Bank, IA, United States), rabbit polyclonal anti-SOX2 (ab15830, 1:150; Abcam, United Kingdom), rabbit polyclonal anti-brain lipid binding protein (BLBP; AB9558, 1:500; Chemicon, Hampshire, United Kingdom), mouse monoclonal anti-VIMENTIN (V6630, 1:40; Sigma-Aldrich), rabbit polyclonal anti-NG2 (AB5320, 1:150; Millipore, United Kingdom), rabbit polyclonal anti-OLIG2 (AB9610, 1:200; Millipore, MA, United States), mouse monoclonal anti-OLIG2 (SAB1404798, 1:50; Sigma-Aldrich), goat polyclonal anti-PROX1 (AF2727, 1:15; R&D Systems), mouse monoclonal anti-CNPase (C5922, 1:500; Sigma-Aldrich), mouse monoclonal anti-adenomatous polyposis coli (CC1 antibody, OP80, 1:1,000; Calbiochem, La Jolla, CA, United States), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; MAB360, 1:800; Chemicon, CA, United States), mouse monoclonal anti-βIII tubulin (Tuj1, 1:100; BabCO, CA, United States), rat monoclonal anti-myelin binding protein (MBP; MAB386, 1:200, Chemicon), and chicken polyclonal anti-neurofilament heavy polypeptide (NFH; ab72996, 1:25,000; Abcam). .. Secondary antibodies raised against the appropriate host species and conjugated with Alexa Fluor 488-, Alexa Fluor 594-, and Alexa Fluor 680 dyes were used to detect primary antibodies (1:500; all from ThermoFisher scientific).

    Binding Assay:

    Article Title: Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells.
    Article Snippet: .. Immunocytochemistry was performed using a standard protocol and the following primary antibodies: mouse monoclonal antiNESTIN (Rat-401, 1:100; Developmental Studies Hybridoma Bank, IA, United States), rabbit polyclonal anti-SOX2 (ab15830, 1:150; Abcam, United Kingdom), rabbit polyclonal anti-brain lipid binding protein (BLBP; AB9558, 1:500; Chemicon, Hampshire, United Kingdom), mouse monoclonal antiVIMENTIN (V6630, 1:40; Sigma-Aldrich), rabbit polyclonal anti-NG2 (AB5320, 1:150; Millipore, United Kingdom), rabbit polyclonal anti-OLIG2 (AB9610, 1:200; Millipore, MA, United States), mouse monoclonal anti-OLIG2 (SAB1404798, 1:50; Sigma-Aldrich), goat polyclonal anti-PROX1 (AF2727, 1:15; R&D Systems), mouse monoclonal anti-CNPase (C5922, 1:500; Sigma-Aldrich), mouse monoclonal anti-adenomatous polyposis coli (CC1 antibody, OP80, 1:1,000; Calbiochem, La Jolla, CA, United States), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; MAB360, 1:800; Chemicon, CA, United States), mouse monoclonal anti-βIII tubulin (Tuj1, 1:100; BabCO, CA, United States), rat monoclonal anti-myelin binding protein (MBP; MAB386, 1:200, Chemicon), and chicken polyclonal anti-neurofilament heavy polypeptide (NFH; ab72996, 1:25,000; Abcam). .. Secondary antibodies raised against the appropriate host species and conjugated with Alexa Fluor 488-, Alexa Fluor 594-, and Alexa Fluor 680 dyes were used to detect primary antibodies (1:500; all from ThermoFisher scientific).

    Article Title: Generation of Multipotential NG2 Progenitors From Mouse Embryonic Stem Cell-Derived Neural Stem Cells
    Article Snippet: .. Immunocytochemistry was performed using a standard protocol and the following primary antibodies: mouse monoclonal anti-NESTIN (Rat-401, 1:100; Developmental Studies Hybridoma Bank, IA, United States), rabbit polyclonal anti-SOX2 (ab15830, 1:150; Abcam, United Kingdom), rabbit polyclonal anti-brain lipid binding protein (BLBP; AB9558, 1:500; Chemicon, Hampshire, United Kingdom), mouse monoclonal anti-VIMENTIN (V6630, 1:40; Sigma-Aldrich), rabbit polyclonal anti-NG2 (AB5320, 1:150; Millipore, United Kingdom), rabbit polyclonal anti-OLIG2 (AB9610, 1:200; Millipore, MA, United States), mouse monoclonal anti-OLIG2 (SAB1404798, 1:50; Sigma-Aldrich), goat polyclonal anti-PROX1 (AF2727, 1:15; R&D Systems), mouse monoclonal anti-CNPase (C5922, 1:500; Sigma-Aldrich), mouse monoclonal anti-adenomatous polyposis coli (CC1 antibody, OP80, 1:1,000; Calbiochem, La Jolla, CA, United States), mouse monoclonal anti-glial fibrillary acidic protein (GFAP; MAB360, 1:800; Chemicon, CA, United States), mouse monoclonal anti-βIII tubulin (Tuj1, 1:100; BabCO, CA, United States), rat monoclonal anti-myelin binding protein (MBP; MAB386, 1:200, Chemicon), and chicken polyclonal anti-neurofilament heavy polypeptide (NFH; ab72996, 1:25,000; Abcam). .. Secondary antibodies raised against the appropriate host species and conjugated with Alexa Fluor 488-, Alexa Fluor 594-, and Alexa Fluor 680 dyes were used to detect primary antibodies (1:500; all from ThermoFisher scientific).



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    Image Search Results


    KEY RESOURCES TABLE

    Journal: Cell reports

    Article Title: Global Analysis of Intercellular Homeodomain Protein Transfer

    doi: 10.1016/j.celrep.2019.06.056

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: Goat polyclonal anti-PROX1 antibody , R and D Systems , RRID: AB_2170716.

    Techniques: Recombinant, In Vitro, Transfection, Protease Inhibitor, Control, Plasmid Preparation, Software, Expressing, Fluorescence